動(dòng)物視頻跟蹤和行為分析系統(tǒng)由高分辨率高幀頻工業(yè)相機(jī)、專業(yè)動(dòng)物行為采集及分析軟件、紅外背光板等組成，無需標(biāo)記即可對(duì)魚類、昆蟲、哺乳動(dòng)物、鳥類的行為進(jìn)行分析。使用時(shí)可直接使用軟件錄制高質(zhì)量的動(dòng)物活動(dòng)視頻并對(duì)視頻中的目標(biāo)動(dòng)物進(jìn)行識(shí)別跟蹤，獲得目標(biāo)動(dòng)物的位置坐標(biāo)（X坐標(biāo)和Y坐標(biāo)；單個(gè)動(dòng)物可做3D跟蹤，即包括XYZ坐標(biāo)）及隨時(shí)間變化的活動(dòng)軌跡。系統(tǒng)可自動(dòng)計(jì)算個(gè)體、處理組、感興趣區(qū)域相關(guān)的20余種行為參數(shù)，并可導(dǎo)出疊加分析數(shù)據(jù)的圖像和視頻以便于使用者進(jìn)行展示。
功能特點(diǎn)
- 采用工業(yè)相機(jī)，高分辨率、高幀頻、超高速傳輸
- 可跟蹤和分析魚類、昆蟲、哺乳動(dòng)物、鳥類及更多
- 基于對(duì)比度或者背景減除法發(fā)現(xiàn)目標(biāo)，無需標(biāo)記物
- 可跟蹤任意大小和形狀的區(qū)域，如迷宮、水箱、呼吸室
- 對(duì)24孔板、48孔板、96孔板進(jìn)行高通量跟蹤和分析
- 3D跟蹤，可導(dǎo)出3D坐標(biāo)和路徑
- 可結(jié)合游泳儀、植入式動(dòng)物體溫心率數(shù)據(jù)采集器等設(shè)備使用
系統(tǒng)配置
- 1彩色工業(yè)攝像機(jī)
- 專業(yè)級(jí)定焦鏡頭
- 專業(yè)動(dòng)物行為采集及分析軟件LoliTrack5
- 紅外背光板：用于透明多孔板和透明魚缸等的底部照明，可消除反射，也可用于黑暗環(huán)境的視頻記錄
- 動(dòng)物活動(dòng)室/池（選配）：定制化水箱/魚缸、活動(dòng)室等
技術(shù)指標(biāo)
- 可自動(dòng)計(jì)算參數(shù)包括：
- 位置坐標(biāo)（包括前、后、中線中心、重心）
- 移動(dòng)距離
- 速度和速率（X方向、Y方向）
- 加速度（X方向、Y方向）
- 移動(dòng)方向
- 身體取向
- 身體彎曲
- 轉(zhuǎn)彎速率
- 移動(dòng)方向和身體取向變化速率
- 活動(dòng)/非活動(dòng)時(shí)間和比例
- 感興趣區(qū)域花費(fèi)時(shí)間和占比
- 目標(biāo)動(dòng)物到感興趣區(qū)域的距離
- 個(gè)體間距離（IID），包括平均、眾數(shù)和最小
- 動(dòng)物行為觀測分析軟件具備多種基于反差法和背景減除法的過濾功能。
- 軟件可跟蹤單個(gè)區(qū)域或多個(gè)區(qū)域內(nèi)的多個(gè)動(dòng)物，即使動(dòng)物具有相同（或不同）的形狀、顏色和大小。
- 可挑選單個(gè)個(gè)體用以跟蹤和分析，而非所有的個(gè)體；可自定義跟蹤時(shí)長，忽略或者只跟蹤某段時(shí)間；具備批量分析功能。
- 軟件可識(shí)別特定身體的點(diǎn)位（如尾、頭、中線中點(diǎn)、重心），跟蹤這些點(diǎn)用以研究運(yùn)動(dòng)學(xué)（如擺尾頻率、身體擺動(dòng)等）或者特定的行為（如身體取向、移動(dòng)方向等）。
- 軟件具備頻率（心率、擺尾頻率等）計(jì)算和容積（心博量等）計(jì)算功能。
- 軟件具備錯(cuò)誤自查功能，并提供手動(dòng)糾錯(cuò)的快捷方式。
- 軟件兼容大部分相機(jī)和視頻格式，具備視頻記錄和編輯工具，具備位置、速度、加速度和轉(zhuǎn)彎速率的熱圖生成功能，并可輸出疊加了分析結(jié)果的視頻和圖片，用戶可自定義Excel數(shù)據(jù)文件輸出內(nèi)容。
- 軟件能夠?qū)蓚€(gè)相機(jī)或者兩個(gè)視頻文件的目標(biāo)動(dòng)物進(jìn)行3D視頻跟蹤和行為分析，導(dǎo)出3D坐標(biāo)和軌跡的3D模型，在其他軟件做進(jìn)一步的可視化和分析。
- 系統(tǒng)可對(duì)24、48或96孔板中的生物進(jìn)行跟蹤和分析，對(duì)其活動(dòng)水平或者運(yùn)動(dòng)進(jìn)行快速篩選評(píng)估（常見于毒理學(xué)或者生物醫(yī)學(xué)檢測）?？奢斎胗脩糇远x的處理描述以幫助分析數(shù)據(jù)。
- 1彩色高清高幀頻工業(yè)攝像機(jī)：緊湊設(shè)計(jì)，分辨率2592 x 1944 (5.04 MP)，幀率48FPS，芯片尺寸1/2.5" (5.702 mm x 4.277 mm)，重量33g。包括USB數(shù)據(jù)線、三腳架適配器、壁掛式支架安裝。
- 專業(yè)級(jí)定焦鏡頭：6mm、8mm、12mm、16mm、25mm、50mm焦距可選。
- 紅外背光板：大小16×16cm至56cm×56cm共6種尺寸可選，波長850nm。
應(yīng)用案例
高濃度人體可卡因代謝物苯甲酰愛康寧（BE）暴露使大型溞個(gè)體的活動(dòng)性顯著降低、游泳速率顯著增大 (Environmental Pollution, 2018)
漸凍癥斑馬魚模型表現(xiàn)出病癥早期階段異常行為增多、晚期階段運(yùn)動(dòng)活性降低的表型(Glia, 2019)
產(chǎn)地：歐洲
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